› 5c6c

X-ray diffraction
2.05Å resolution

PKG II's Amino Terminal Cyclic Nucleotide Binding Domain (CNB-A) in a complex with cAMP

Released:
DOI: 10.2210/pdb5c6c/pdb
PDB entry 5c6c coloured by chain, front view.
PDB entry 5c6c coloured by chain, side view.
PDB entry 5c6c coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Basis of Cyclic Nucleotide Selectivity in cGMP-dependent Protein Kinase II.
Campbell JC, Kim JJ, Li KY, Huang GY, Reger AS, Matsuda S, Sankaran B, Link TM, Yuasa K, Ladbury JE, Casteel DE, Kim C
J Biol Chem 291 5623-5633 (2016)
PMID: 26769964

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171613 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cGMP-dependent protein kinase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 143 amino acids
Theoretical weight: 16.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13237 (Residues: 137-277; Coverage: 19%)
Gene names: PRKG2, PRKGR2
Sequence domains: Cyclic nucleotide-binding domain
Structure domains: Jelly Rolls

Ligands and Environments

6 bound ligands:
Ligand CD 6 x CD
Ligand CMP 2 x CMP
Ligand CO 4 x CO
Ligand CA 2 x CA
Ligand EDO 1 x EDO
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 46.71Å b: 63.76Å c: 99.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.186 0.243
Expression system: Escherichia coli

Quick links

Citations

9 citation in other articles

Structures of the cGMP-dependent protein kinase in malaria parasites reveal a unique structural relay mechanism for activation.
El Bakkouri et al. (2019)
8 more

3 mentions without citation

Cyclic nucleotide selectivity of protein kinase G isozymes.
Kim et al. (2021)
2 more