Function and Biology Details
Reactions catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Sequence domain:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187974 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin carboxyl-terminal hydrolase 7
Molecule details ›
Chains: A, B
Length: 322 amino acids
Theoretical weight: 37.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: ICP0-binding domain of Ubiquitin-specific protease 7
Length: 322 amino acids
Theoretical weight: 37.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical:
Q93009 (Residues: 561-881; Coverage: 29%)
Sequence domains: ICP0-binding domain of Ubiquitin-specific protease 7
E3 ubiquitin-protein ligase UHRF1
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
APS BEAMLINE 24-ID-E
Spacegroup:
C2
Expression system: Escherichia coli
