› 5c6h

X-ray diffraction
2.05Å resolution

Mcl-1 complexed with Mule

Released:
DOI: 10.2210/pdb5c6h/pdb
PDB entry 5c6h coloured by chain, front view.
PDB entry 5c6h coloured by chain, side view.
PDB entry 5c6h coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Song T, Wang Z, Ji F, Chai G, Liu Y, Li X, Li Z, Fan Y, Zhang Z

Function and Biology Details

Reaction catalysed: 
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero 24-mer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170536 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Induced myeloid leukemia cell differentiation protein Mcl-1 Chains: A, C, E, G, I, K, M, O, Q, S, U, W
Molecule details ›
Chains: A, C, E, G, I, K, M, O, Q, S, U, W
Length: 157 amino acids
Theoretical weight: 17.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q07820 (Residues: 171-327; Coverage: 45%)
Gene names: BCL2L3, MCL1
Sequence domains: Apoptosis regulator proteins, Bcl-2 family
Structure domains: Blc2-like
E3 ubiquitin-protein ligase HUWE1 Chains: B, D, F, H, J, L, N, P, R, T, V, X
Molecule details ›
Chains: B, D, F, H, J, L, N, P, R, T, V, X
Length: 26 amino acids
Theoretical weight: 2.97 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q7Z6Z7 (Residues: 1969-1994; Coverage: 1%)
Gene names: HSPC272, HUWE1, KIAA0312, KIAA1578, UREB1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P1
Unit cell:
a: 31.79Å b: 114.24Å c: 135.98Å
α: 90.12° β: 92.32° γ: 90.01°
R-values:
R R work R free
0.297 0.295 0.346
Expression systems:
  • Escherichia coli
  • Not provided

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