› 5c7o

X-ray diffraction
1.73Å resolution

Structure of human testis-specific glyceraldehyde-3-phosphate dehydrogenase holo form with NAD+

Released:
DOI: 10.2210/pdb5c7o/pdb
PDB entry 5c7o coloured by chain, front view.
PDB entry 5c7o coloured by chain, side view.
PDB entry 5c7o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural analyses to identify selective inhibitors of glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme.
Danshina PV, Qu W, Temple BR, Rojas RJ, Miley MJ, Machius M, Betts L, O'Brien DA
Mol Hum Reprod 22 410-26 (2016)
PMID: 26921398

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127002 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyceraldehyde-3-phosphate dehydrogenase, testis-specific Chains: O, P
Molecule details ›
Chains: O, P
Length: 335 amino acids
Theoretical weight: 36.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14556 (Residues: 74-407; Coverage: 82%)
Gene names: GAPD2, GAPDH2, GAPDHS, GAPDS, HSD-35, HSD35
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand OCS 2 x OCS

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 144.442Å b: 71.741Å c: 80.844Å
α: 90° β: 123.01° γ: 90°
R-values:
R R work R free
0.138 0.137 0.166
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The control of male fertility by spermatid-specific factors: searching for contraceptive targets from spermatozoon's head to tail.
Chen et al. (2016)
8 other articles

3 mentions without citation

Structural analyses to identify selective inhibitors of glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme.
Danshina et al. (2016)
2 more