› 5cb1

X-ray diffraction
3.3Å resolution

Apo enzyme of human Polymerase lambda

Released:
DOI: 10.2210/pdb5cb1/pdb
PDB entry 5cb1 coloured by chain, front view.
PDB entry 5cb1 coloured by chain, side view.
PDB entry 5cb1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Mechanism for the Fidelity Modulation of DNA Polymerase λ.
Liu MS, Tsai HY, Liu XX, Ho MC, Wu WJ, Tsai MD
J Am Chem Soc 138 2389-98 (2016)
PMID: 26836966

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193722 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase lambda Chains: A, B
Molecule details ›
Chains: A, B
Length: 326 amino acids
Theoretical weight: 36.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UGP5 (Residues: 250-575; Coverage: 57%)
Gene name: POLL
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: I4122
Unit cell:
a: 205.285Å b: 205.285Å c: 111.395Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.23 0.264
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Nonhomologous DNA end-joining for repair of DNA double-strand breaks.
Pannunzio et al. (2018)
1 more

2 mentions without citation

Structure and function relationships in mammalian DNA polymerases.
Hoitsma et al. (2020)
1 more