› 5cil

X-ray diffraction
1.81Å resolution

Crystal Structure of non-neutralizing version of 4E10 (WDWD) with epitope bound

Released:
DOI: 10.2210/pdb5cil/pdb
PDB entry 5cil coloured by chain, front view.
PDB entry 5cil coloured by chain, side view.
PDB entry 5cil coloured by chain, top view.
Source organisms:
Primary publication:
Structural and Thermodynamic Basis of Epitope Binding by Neutralizing and Nonneutralizing Forms of the Anti-HIV-1 Antibody 4E10.
Rujas E, Gulzar N, Morante K, Tsumoto K, Scott JK, Nieva JL, Caaveiro JM
J Virol 89 11975-89 (2015)
PMID: 26378169

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235030 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
FAB 4E10 HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 226 amino acids
Theoretical weight: 23.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
FAB 4E10 LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 212 amino acids
Theoretical weight: 23.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
Transmembrane protein gp41 Chain: P
Molecule details ›
Chain: P
Length: 16 amino acids
Theoretical weight: 2.19 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Not provided
UniProt:
  • Canonical: P04578 (Residues: 671-683; Coverage: 2%)
Gene name: env

Ligands and Environments

3 bound ligands:
Ligand GOL 2 x GOL
Ligand CL 2 x CL
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: C2
Unit cell:
a: 157.3Å b: 44.89Å c: 86.16Å
α: 90° β: 114.53° γ: 90°
R-values:
R R work R free
0.164 0.163 0.188
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

HIV-1 Entry and Prospects for Protecting against Infection.
Bruxelle et al. (2021)
16 other articles

1 mention without citation

Structural and Thermodynamic Basis of Epitope Binding by Neutralizing and Nonneutralizing Forms of the Anti-HIV-1 Antibody 4E10.
Rujas et al. (2015)