› 5cj2

X-ray diffraction
1.75Å resolution

Ran GDP Y39A mutant triclinic crystal form

Released:
DOI: 10.2210/pdb5cj2/pdb
PDB entry 5cj2 coloured by chain, front view.
PDB entry 5cj2 coloured by chain, side view.
PDB entry 5cj2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Catalysis of GTP hydrolysis by small GTPases at atomic detail by integration of X-ray crystallography, experimental, and theoretical IR spectroscopy.
Rudack T, Jenrich S, Brucker S, Vetter IR, Gerwert K, Kötting C
J Biol Chem 290 24079-90 (2015)
PMID: 26272610

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158719 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTP-binding nuclear protein Ran Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 216 amino acids
Theoretical weight: 24.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62826 (Residues: 1-216; Coverage: 100%)
Gene names: ARA24, OK/SW-cl.81, RAN
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand GDP 8 x GDP
Ligand MG 12 x MG
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P1
Unit cell:
a: 59.19Å b: 80.73Å c: 91.65Å
α: 97.66° β: 90° γ: 90.01°
R-values:
R R work R free
0.199 0.197 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Invited review: Small GTPases and their GAPs.
Mishra et al. (2016)
2 more

1 mention without citation

Catalysis of GTP hydrolysis by small GTPases at atomic detail by integration of X-ray crystallography, experimental, and theoretical IR spectroscopy.
Rudack et al. (2015)