› 5cl1

X-ray diffraction
3.8Å resolution

Complex structure of Norrin with human Frizzled 4

Released:
DOI: 10.2210/pdb5cl1/pdb
PDB entry 5cl1 coloured by chain, front view.
PDB entry 5cl1 coloured by chain, side view.
PDB entry 5cl1 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of the Norrin-Frizzled 4 interaction.
Shen G, Ke J, Wang Z, Cheng Z, Gu X, Wei Y, Melcher K, Xu HE, Xu W
Cell Res 25 1078-81 (2015)
PMID: 26227961

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142651 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein; Norrin Chains: A, B
Molecule details ›
Chains: A, B
Length: 483 amino acids
Theoretical weight: 53.66 KDa
Source organisms: Expression system: Trichoplusia ni
UniProt:
  • Canonical: P0AEY0 (Residues: 26-392; Coverage: 99%)
  • Canonical: Q00604 (Residues: 31-133; Coverage: 95%)
Gene names: ECs5017, EVR2, NDP, Z5632, malE
Sequence domains:
Frizzled-4 Chains: C, D
Molecule details ›
Chains: C, D
Length: 131 amino acids
Theoretical weight: 15.1 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9ULV1 (Residues: 32-160; Coverage: 24%)
Gene name: FZD4
Sequence domains: Fz domain
Structure domains: Frizzled cysteine-rich domain

Ligands and Environments

1 bound ligand:
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P21212
Unit cell:
a: 143.291Å b: 150.589Å c: 92.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.264 0.262 0.31
Expression system: Trichoplusia ni

Quick links

Citations

5 review citations

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
4 more

5 mentions without citation

Assembly and architecture of the Wnt/β-catenin signalosome at the membrane.
DeBruine et al. (2017)
4 more