› 5cll

X-ray diffraction
2.45Å resolution

Truncated Ran wild type in complex with GDP-BeF and RanBD1

Released:
DOI: 10.2210/pdb5cll/pdb
PDB entry 5cll coloured by chain, front view.
PDB entry 5cll coloured by chain, side view.
PDB entry 5cll coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Catalysis of GTP hydrolysis by small GTPases at atomic detail by integration of X-ray crystallography, experimental, and theoretical IR spectroscopy.
Rudack T, Jenrich S, Brucker S, Vetter IR, Gerwert K, Kötting C
J Biol Chem 290 24079-90 (2015)
PMID: 26272610

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156036 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GTP-binding nuclear protein Ran Chains: A, C
Molecule details ›
Chains: A, C
Length: 191 amino acids
Theoretical weight: 21.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62826 (Residues: 1-191; Coverage: 88%)
Gene names: ARA24, OK/SW-cl.81, RAN
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
E3 SUMO-protein ligase RanBP2 Chains: B, D
Molecule details ›
Chains: B, D
Length: 167 amino acids
Theoretical weight: 18.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49792 (Residues: 1155-1321; Coverage: 5%)
Gene names: NUP358, RANBP2
Sequence domains: RanBP1 domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

3 bound ligands:
Ligand GDP 2 x GDP
Ligand BEF 2 x BEF
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 50.6Å b: 55.78Å c: 122.93Å
α: 90° β: 90.05° γ: 90°
R-values:
R R work R free
0.237 0.235 0.272
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Invited review: Small GTPases and their GAPs.
Mishra et al. (2016)
2 more

2 mentions without citation

Catalysis of GTP hydrolysis by small GTPases at atomic detail by integration of X-ray crystallography, experimental, and theoretical IR spectroscopy.
Rudack et al. (2015)
1 more