› 5clw

X-ray diffraction
2.8Å resolution

Crystal structure of human glycogen branching enzyme (GBE1) in complex with maltoheptaose

Released:
DOI: 10.2210/pdb5clw/pdb
PDB entry 5clw coloured by chain, front view.
PDB entry 5clw coloured by chain, side view.
PDB entry 5clw coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Krojer T, Froese DS, Goubin S, Strain-Damerell C, Mahajan P, Burgess-Brown N, von Delft F, Bountra C, Arrowsmith CH, Edwards A, Yue W, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170031 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
1,4-alpha-glucan-branching enzyme Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 668 amino acids
Theoretical weight: 77.04 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: Q04446 (Residues: 38-700; Coverage: 94%)
Gene name: GBE1
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2221
Unit cell:
a: 116.689Å b: 164.536Å c: 313.208Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.231
Expression system: unidentified baculovirus

Quick links