› 5coc

X-ray diffraction
2.67Å resolution

Fusion protein of human calmodulin and B4 domain of protein A from staphylococcal aureus

Released:
DOI: 10.2210/pdb5coc/pdb
PDB entry 5coc coloured by chain, front view.
PDB entry 5coc coloured by chain, side view.
PDB entry 5coc coloured by chain, top view.
Source organisms:
Primary publication:
Connecting two proteins using a fusion alpha helix stabilized by a chemical cross linker.
Jeong WH, Lee H, Song DH, Eom JH, Kim SC, Lee HS, Lee H, Lee JO
Nat Commun 7 11031 (2016)
PMID: 26980593

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143906 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calmodulin-1; Immunoglobulin G-binding protein A Chain: A
Molecule details ›
Chain: A
Length: 130 amino acids
Theoretical weight: 14.55 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P38507 (Residues: 213-275; Coverage: 12%)
  • Canonical: P0DP23 (Residues: 7-78; Coverage: 48%)
Gene names: CALM, CALM1, CAM, CAM1, spa
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1)
Spacegroup: I41
Unit cell:
a: 98.807Å b: 98.807Å c: 31.675Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.206 0.259
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Geometric Principles for Designing Highly Symmetric Self-Assembling Protein Nanomaterials.
Yeates TO. (2017)
2 more

1 mention without citation

Connecting two proteins using a fusion alpha helix stabilized by a chemical cross linker.
Jeong et al. (2016)