› 5cru

X-ray diffraction
2.4Å resolution

Crystal structure of the Bro domain of HD-PTP

Released:
DOI: 10.2210/pdb5cru/pdb
PDB entry 5cru coloured by chain, front view.
PDB entry 5cru coloured by chain, side view.
PDB entry 5cru coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural Study of the HD-PTP Bro1 Domain in a Complex with the Core Region of STAM2, a Subunit of ESCRT-0.
Lee J, Oh KJ, Lee D, Kim BY, Choi JS, Ku B, Kim SJ
PLoS One 11 e0149113 (2016)
PMID: 26866605

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190589 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 23 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 361 amino acids
Theoretical weight: 40.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H3S7 (Residues: 1-361; Coverage: 22%)
Gene names: KIAA1471, PTPN23
Sequence domains: BRO1-like domain
Structure domains: alix/aip1 like domains

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 5C (4A)
Spacegroup: P1
Unit cell:
a: 64.99Å b: 66.874Å c: 82.424Å
α: 89.95° β: 89.94° γ: 89.84°
R-values:
R R work R free
0.222 0.22 0.259
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Cellular Functions and Molecular Mechanisms of the ESCRT Membrane-Scission Machinery.
Christ et al. (2017)
3 more

2 mentions without citation

Structural Study of the HD-PTP Bro1 Domain in a Complex with the Core Region of STAM2, a Subunit of ESCRT-0.
Lee et al. (2016)
1 more