› 5cuh

X-ray diffraction
1.83Å resolution

Crystal structure MMP-9 complexes with a constrained hydroxamate based inhibitor LT4

Released:
DOI: 10.2210/pdb5cuh/pdb
PDB entry 5cuh coloured by chain, front view.
PDB entry 5cuh coloured by chain, side view.
PDB entry 5cuh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Discovery of a new selective inhibitor of A Disintegrin And Metalloprotease 10 (ADAM-10) able to reduce the shedding of NKG2D ligands in Hodgkin's lymphoma cell models.
Camodeca C, Nuti E, Tepshi L, Boero S, Tuccinardi T, Stura EA, Poggi A, Zocchi MR, Rossello A
Eur J Med Chem 111 193-201 (2016)
PMID: 26871660

Function and Biology Details

Reaction catalysed: 
Cleavage of gelatin types I and V and collagen types IV and V.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147085 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
67 kDa matrix metalloproteinase-9 Chains: A, B
Molecule details ›
Chains: A, B
Length: 164 amino acids
Theoretical weight: 18.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14780 (Residues: 107-444; Coverage: 24%)
Gene names: CLG4B, MMP9
Sequence domains: Matrixin
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

6 bound ligands:
Ligand ZN 4 x ZN
Ligand CA 6 x CA
Ligand LTQ 2 x LTQ
Ligand EDO 4 x EDO
Ligand PGO 2 x PGO
Ligand DMS 1 x DMS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P21212
Unit cell:
a: 74.68Å b: 97.79Å c: 43.49Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.214
Expression system: Escherichia coli

Quick links

Citations

9 review citations

The emerging role of ADAM metalloproteinases in immunity.
Lambrecht et al. (2018)
8 more

10 mentions without citation

Molecular basis for the mechanism of action of an anti-TACE antibody.
Peng et al. (2016)
9 more