› 5cus

X-ray diffraction
3.2Å resolution

Crystal Structure of sErbB3-Fab3379 Complex

Released:
DOI: 10.2210/pdb5cus/pdb
PDB entry 5cus coloured by chain, front view.
PDB entry 5cus coloured by chain, side view.
PDB entry 5cus coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Inhibition of ErbB3 by a monoclonal antibody that locks the extracellular domain in an inactive configuration.
Lee S, Greenlee EB, Amick JR, Ligon GF, Lillquist JS, Natoli EJ, Hadari Y, Alvarado D, Schlessinger J
Proc Natl Acad Sci U S A 112 13225-30 (2015)
PMID: 26460020

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-229914 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Receptor tyrosine-protein kinase erbB-3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 627 amino acids
Theoretical weight: 69.22 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P21860 (Residues: 20-641; Coverage: 47%)
Gene names: ERBB3, HER3
Sequence domains:
Structure domains:
Ig-like domain-containing protein Chains: H, I, J, K
Molecule details ›
Chains: H, I, J, K
Length: 221 amino acids
Theoretical weight: 23.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: S6B291 (Residues: 20-116, 117-239; Coverage: 49%)
Sequence domains:
Structure domains: Immunoglobulins
Fab LC region of KTN3379 Chains: L, M, N, O
Molecule details ›
Chains: L, M, N, O
Length: 216 amino acids
Theoretical weight: 22.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand NAG 11 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P1
Unit cell:
a: 84.378Å b: 127.141Å c: 138.078Å
α: 87.1° β: 85.54° γ: 89.92°
R-values:
R R work R free
0.254 0.252 0.278
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

A comprehensive review of heregulins, HER3, and HER4 as potential therapeutic targets in cancer.
Mota et al. (2017)
3 more

3 mentions without citation

Insulin and epidermal growth factor receptor family members share parallel activation mechanisms.
Ferguson et al. (2020)
2 more