› 5czx

X-ray diffraction
2.1Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-230404 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Notch 3 extracellular truncation Chains: A, B
Molecule details ›
Chains: A, B
Length: 271 amino acids
Theoretical weight: 30.1 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9UM47 (Residues: 1378-1640; Coverage: 12%)
Gene name: NOTCH3
Sequence domains:
Structure domains:
20358 Fab heavy chain Chains: C, H
Molecule details ›
Chains: C, H
Length: 226 amino acids
Theoretical weight: 24.12 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins
20358 Fab light chain Chains: D, L
Molecule details ›
Chains: D, L
Length: 214 amino acids
Theoretical weight: 23.39 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

4 bound ligands:
Ligand NAG 2 x NAG
Ligand CA 6 x CA
Ligand EDO 10 x EDO
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 88.34Å b: 123.86Å c: 150.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.19 0.226
Expression system: Homo sapiens

Quick links

Citations

26 review citations

Notch Signaling in Development, Tissue Homeostasis, and Disease.
Siebel et al. (2017)
25 more

4 mentions without citation

Characterization of activating mutations of NOTCH3 in T-cell acute lymphoblastic leukemia and anti-leukemic activity of NOTCH3 inhibitory antibodies.
Bernasconi-Elias et al. (2016)
3 more