› 5d1y

X-ray diffraction
9Å resolution

Low resolution crystal structure of human ribonucleotide reductase alpha6 hexamer in complex with dATP

Released:
DOI: 10.2210/pdb5d1y/pdb
PDB entry 5d1y coloured by chain, front view.
PDB entry 5d1y coloured by chain, side view.
PDB entry 5d1y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Allosteric Inhibition of Human Ribonucleotide Reductase by dATP Entails the Stabilization of a Hexamer.
Ando N, Li H, Brignole EJ, Thompson S, McLaughlin MI, Page JE, Asturias FJ, Stubbe J, Drennan CL
Biochemistry 55 373-81 (2016)
PMID: 26727048

Function and Biology Details

Reaction catalysed: 
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150119 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonucleoside-diphosphate reductase large subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 812 amino acids
Theoretical weight: 92.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23921 (Residues: 1-792; Coverage: 100%)
Gene names: RR1, RRM1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P4132
Unit cell:
a: 356.01Å b: 356.01Å c: 356.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.407 0.405 0.426
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

Ribonucleotide Reductases: Structure, Chemistry, and Metabolism Suggest New Therapeutic Targets.
Greene et al. (2020)
5 more

3 mentions without citation

X-ray Scattering Studies of Protein Structural Dynamics.
Meisburger et al. (2017)
2 more