› 5d27

X-ray diffraction
1.92Å resolution

Crystal Structure of the P-Rex1 PH domain

Released:
DOI: 10.2210/pdb5d27/pdb
PDB entry 5d27 coloured by chain, front view.
PDB entry 5d27 coloured by chain, side view.
PDB entry 5d27 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and Biochemical Characterization of the Catalytic Core of the Metastatic Factor P-Rex1 and Its Regulation by PtdIns(3,4,5)P3.
Cash JN, Davis EM, Tesmer JJG
Structure 24 730-740 (2016)
PMID: 27150042

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186195 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein Chain: A
Molecule details ›
Chain: A
Length: 167 amino acids
Theoretical weight: 19.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8TCU6 (Residues: 245-408; Coverage: 10%)
Gene names: KIAA1415, PREX1
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

1 bound ligand:
Ligand NI 1 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P3212
Unit cell:
a: 39.897Å b: 39.897Å c: 187.269Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.188 0.232
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Rac1 Signaling: From Intestinal Homeostasis to Colorectal Cancer Metastasis.
Kotelevets et al. (2020)
4 more

3 mentions without citation

Structural and Biochemical Characterization of the Catalytic Core of the Metastatic Factor P-Rex1 and Its Regulation by PtdIns(3,4,5)P3.
Cash et al. (2016)
2 more