5d2n

X-ray diffraction
2.1Å resolution

Crystal structure of C25-NLV-HLA-A2 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-229833 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
C25 alpha Chains: C, D
Molecule details ›
Chains: C, D
Length: 203 amino acids
Theoretical weight: 22.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
C25 beta Chains: E, F
Molecule details ›
Chains: E, F
Length: 247 amino acids
Theoretical weight: 27.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
HLA class I histocompatibility antigen, A alpha chain Chains: A, H
Molecule details ›
Chains: A, H
Length: 276 amino acids
Theoretical weight: 31.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04439 (Residues: 25-299; Coverage: 81%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, L
Molecule details ›
Chains: B, L
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
65 kDa phosphoprotein Chains: G, I
Molecule details ›
Chains: G, I
Length: 9 amino acids
Theoretical weight: 943 Da
Source organism: Cytomegalovirus
Expression system: Not provided
UniProt:
  • Canonical: P18139 (Residues: 485-493; Coverage: 2%)
Gene name: UL83

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 84.307Å b: 124.874Å c: 193.843Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.204 0.253
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided