› 5d5a

X-ray diffraction
2.48Å resolution

In meso in situ serial X-ray crystallography structure of the Beta2-adrenergic receptor at 100 K

Released:
DOI: 10.2210/pdb5d5a/pdb
PDB entry 5d5a coloured by chain, front view.
PDB entry 5d5a coloured by chain, side view.
PDB entry 5d5a coloured by chain, top view.
Source organisms:
Primary publication:
In meso in situ serial X-ray crystallography of soluble and membrane proteins at cryogenic temperatures.
Huang CY, Olieric V, Ma P, Howe N, Vogeley L, Liu X, Warshamanage R, Weinert T, Panepucci E, Kobilka B, Diederichs K, Wang M, Caffrey M
Acta Crystallogr D Struct Biol 72 93-112 (2016)
PMID: 26894538

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132941 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-2 adrenergic receptor; Endolysin Chain: A
Molecule details ›
Chain: A
Length: 500 amino acids
Theoretical weight: 56.6 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P07550 (Residues: 1-230, 264-365; Coverage: 80%)
  • Canonical: P00720 (Residues: 2-162; Coverage: 98%)
Gene names: ADRB2, ADRB2R, B2AR, E
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GLC
Carbohydrate polymer
7 bound ligands:
Ligand CAU 1 x CAU
Ligand BU1 2 x BU1
Ligand ACM 1 x ACM
Ligand CLR 3 x CLR
Ligand PLM 1 x PLM
Ligand 12P 1 x 12P
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2
Unit cell:
a: 107Å b: 170Å c: 40.5Å
α: 90° β: 106.25° γ: 90°
R-values:
R R work R free
0.214 0.212 0.262
Expression system: Spodoptera frugiperda

Quick links

Citations

3 review citations

Structure-Function Relationships of Olfactory and Taste Receptors.
Behrens et al. (2018)
2 more

14 mentions without citation

G protein-coupled receptors: structure- and function-based drug discovery.
Yang et al. (2021)
13 more