5d5v

X-ray diffraction
2.55Å resolution

Crystal structure of human Hsf1 with Satellite III repeat DNA

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-118540 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Heat shock factor protein 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 131 amino acids
Theoretical weight: 14.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q00613 (Residues: 1-120; Coverage: 23%)
Gene names: HSF1, HSTF1
Sequence domains: HSF-type DNA-binding
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA Chain: A
Molecule details ›
Chain: A
Length: 12 nucleotides
Theoretical weight: 3.75 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA Chain: C
Molecule details ›
Chain: C
Length: 12 nucleotides
Theoretical weight: 3.57 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 104.813Å b: 112.678Å c: 39.865Å
α: 90° β: 92.56° γ: 90°
R-values:
R R work R free
0.192 0.19 0.234
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided