› 5d65

X-ray diffraction
3.1Å resolution

X-RAY STRUCTURE OF MACROPHAGE INFLAMMATORY PROTEIN-1 ALPHA (CCL3) WITH HEPARIN COMPLEX

Released:
DOI: 10.2210/pdb5d65/pdb
PDB entry 5d65 coloured by chain, front view.
PDB entry 5d65 coloured by chain, side view.
PDB entry 5d65 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for oligomerization and glycosaminoglycan binding of CCL5 and CCL3.
Liang WG, Triandafillou CG, Huang TY, Zulueta MM, Banerjee S, Dinner AR, Hung SC, Tang WJ
Proc Natl Acad Sci U S A 113 5000-5 (2016)
PMID: 27091995

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145191 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
C-C motif chemokine 3 Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 70 amino acids
Theoretical weight: 7.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10147 (Residues: 23-92; Coverage: 100%)
Gene names: CCL3, G0S19-1, MIP1A, SCYA3
Sequence domains: Small cytokines (intecrine/chemokine), interleukin-8 like
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

Carbohydrate polymer : NEW Components: IDS, SGN
Carbohydrate polymer
3 bound ligands:
Ligand BGC 9 x BGC
Ligand CL 2 x CL
Ligand GLC 1 x GLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P6222
Unit cell:
a: 181.061Å b: 181.061Å c: 77.378Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.215 0.214 0.243
Expression system: Escherichia coli

Quick links

Citations

17 review citations

A guide to chemokines and their receptors.
Hughes et al. (2018)
16 more

4 mentions without citation

The marriage of chemokines and galectins as functional heterodimers.
von Hundelshausen et al. (2021)
3 more