› 5d6b

X-ray diffraction
2.1Å resolution

Human fumarase (wild type)

Released:
DOI: 10.2210/pdb5d6b/pdb
PDB entry 5d6b coloured by chain, front view.
PDB entry 5d6b coloured by chain, side view.
PDB entry 5d6b coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of recombinant human fumarase.
Pereira de Pádua RA, Nonato MC
Acta Crystallogr F Struct Biol Commun 70 120-2 (2014)
PMID: 24419633

Function and Biology Details

Reaction catalysed: 
(S)-malate = fumarate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139859 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarate hydratase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 462 amino acids
Theoretical weight: 49.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07954 (Residues: 49-510; Coverage: 91%)
Gene name: FH
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: C222
Unit cell:
a: 124.42Å b: 147.15Å c: 57.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.18 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Biochemical Characterization of Two Clinically-Relevant Human Fumarase Variants Defective for Oligomerization.
Bulku et al. (2018)
4 other articles

2 mentions without citation

Targeted sequencing of FH-deficient uterine leiomyomas reveals biallelic inactivating somatic fumarase variants and allows characterization of missense variants.
Popp et al. (2020)
1 more