› 5d72

X-ray diffraction
2.6Å resolution

Crystal structure of MOR04252, a neutralizing anti-human GM-CSF antibody Fab fragment in complex with human GM-CSF

Released:
DOI: 10.2210/pdb5d72/pdb
PDB entry 5d72 coloured by chain, front view.
PDB entry 5d72 coloured by chain, side view.
PDB entry 5d72 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis of in vitro affinity maturation and functional evolution of a neutralizing anti-human GM-CSF antibody.
Eylenstein R, Weinfurtner D, Härtle S, Strohner R, Böttcher J, Augustin M, Ostendorp R, Steidl S
MAbs 8 176-86 (2016)
PMID: 26406987

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208982 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Granulocyte-macrophage colony-stimulating factor Chains: A, B
Molecule details ›
Chains: A, B
Length: 141 amino acids
Theoretical weight: 15.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04141 (Residues: 1-127; Coverage: 87%)
Gene names: CSF2, GMCSF
Sequence domains: Granulocyte-macrophage colony-stimulating factor
Structure domains: Growth Hormone; Chain: A;
Immunglobulin G1 Fab fragment, heavy chain Chains: H, M
Molecule details ›
Chains: H, M
Length: 238 amino acids
Theoretical weight: 25.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Immunglobulin G1 Fab fragment, light chain Chains: L, N
Molecule details ›
Chains: L, N
Length: 209 amino acids
Theoretical weight: 22.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 71.822Å b: 97.853Å c: 192.667Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.232 0.266
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Crystal structure of the Fab region of a neutralizing antibody against granulocyte-macrophage colony-stimulating factor.
Angkawidjaja et al. (2019)
5 more

2 mentions without citation

Molecular basis of in vitro affinity maturation and functional evolution of a neutralizing anti-human GM-CSF antibody.
Eylenstein et al. (2016)
1 more