› 5ddo

X-ray diffraction
3.1Å resolution

Structural and Dynamic Basis for Low Affinity-High Selectivity Binding of L-glutamine by the Gln-riboswitch

Released:
DOI: 10.2210/pdb5ddo/pdb
PDB entry 5ddo coloured by chain, front view.
PDB entry 5ddo coloured by chain, side view.
PDB entry 5ddo coloured by chain, top view.
Source organisms:
Primary publication:
Structural and Dynamic Basis for Low-Affinity, High-Selectivity Binding of L-Glutamine by the Glutamine Riboswitch.
Ren A, Xue Y, Peselis A, Serganov A, Al-Hashimi HM, Patel DJ
Cell Rep 13 1800-13 (2015)
PMID: 26655897

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140505 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
U1 small nuclear ribonucleoprotein A Chains: C, G
Molecule details ›
Chains: C, G
Length: 97 amino acids
Theoretical weight: 11.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09012 (Residues: 2-98; Coverage: 34%)
Gene name: SNRPA
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
L-glutamine riboswitch (58-MER) Chains: A, B
Molecule details ›
Chains: A, B
Length: 61 nucleotides
Theoretical weight: 19.72 KDa
Source organism: Synechococcus elongatus

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 80.84Å b: 99.652Å c: 88.461Å
α: 90° β: 99.05° γ: 90°
R-values:
R R work R free
0.23 0.227 0.289
Expression system: Escherichia coli

Quick links

Citations

8 review citations

RNA Structure: Advances and Assessment of 3D Structure Prediction.
Miao et al. (2017)
7 more

9 mentions without citation

RNA-Puzzles Round III: 3D RNA structure prediction of five riboswitches and one ribozyme.
Miao et al. (2017)
8 more