› 5ddq

X-ray diffraction
2.4Å resolution

L-glutamine riboswitch bound with L-glutamine soaked with Mn2+

Released:
DOI: 10.2210/pdb5ddq/pdb
PDB entry 5ddq coloured by chain, front view.
PDB entry 5ddq coloured by chain, side view.
PDB entry 5ddq coloured by chain, top view.
Source organisms:
Primary publication:
Structural and Dynamic Basis for Low-Affinity, High-Selectivity Binding of L-Glutamine by the Glutamine Riboswitch.
Ren A, Xue Y, Peselis A, Serganov A, Al-Hashimi HM, Patel DJ
Cell Rep 13 1800-13 (2015)
PMID: 26655897

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140507 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
U1 small nuclear ribonucleoprotein A Chains: C, D
Molecule details ›
Chains: C, D
Length: 97 amino acids
Theoretical weight: 11.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09012 (Residues: 2-98; Coverage: 34%)
Gene name: SNRPA
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
L-glutamine riboswitch RNA (61-MER) Chains: A, B
Molecule details ›
Chains: A, B
Length: 61 nucleotides
Theoretical weight: 19.74 KDa
Source organism: Synechococcus elongatus

Ligands and Environments

4 bound ligands:
Ligand GLN 2 x GLN
Ligand MN 5 x MN
Ligand MG 3 x MG
Ligand NA 12 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 60.605Å b: 85.701Å c: 60.889Å
α: 90° β: 99.72° γ: 90°
R-values:
R R work R free
0.203 0.197 0.257
Expression system: Escherichia coli

Quick links

Citations

8 review citations

RNA Structure: Advances and Assessment of 3D Structure Prediction.
Miao et al. (2017)
7 more

2 mentions without citation

RNA-Puzzles Round III: 3D RNA structure prediction of five riboswitches and one ribozyme.
Miao et al. (2017)
1 more