› 5dgo

X-ray diffraction
2.1Å resolution

Crystal structure of cell division cycle protein 45 (Cdc45)

Released:
DOI: 10.2210/pdb5dgo/pdb
PDB entry 5dgo coloured by chain, front view.
PDB entry 5dgo coloured by chain, side view.
PDB entry 5dgo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human Cdc45 and implications for CMG helicase function.
Simon AC, Sannino V, Costanzo V, Pellegrini L
Nat Commun 7 11638 (2016)
PMID: 27189187

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130955 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cell division control protein 45 homolog Chain: A
Molecule details ›
Chain: A
Length: 555 amino acids
Theoretical weight: 64.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O75419 (Residues: 1-566; Coverage: 98%)
Gene names: CDC45, CDC45L, CDC45L2, UNQ374/PRO710
Sequence domains: CDC45-like protein

Ligands and Environments

2 bound ligands:
Ligand EDO 6 x EDO
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P3121
Unit cell:
a: 112.795Å b: 112.795Å c: 143.06Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.169 0.168 0.195
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Eukaryotic DNA Replication Fork.
Burgers et al. (2017)
9 more

11 mentions without citation

Structural insights into Cdc45 function: was there a nuclease at the heart of the ancestral replisome?
Pellegrini L. (2017)
10 more