› 5dh3

X-ray diffraction
2.47Å resolution

Crystal structure of MST2 in complex with XMU-MP-1

Released:
DOI: 10.2210/pdb5dh3/pdb
PDB entry 5dh3 coloured by chain, front view.
PDB entry 5dh3 coloured by chain, side view.
PDB entry 5dh3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Pharmacological targeting of kinases MST1 and MST2 augments tissue repair and regeneration.
Fan F, He Z, Kong LL, Chen Q, Yuan Q, Zhang S, Ye J, Liu H, Sun X, Geng J, Yuan L, Hong L, Xiao C, Zhang W, Sun X, Li Y, Wang P, Huang L, Wu X, Ji Z, Wu Q, Xia NS, Gray NS, Chen L, Yun CH, Deng X, Zhou D
Sci Transl Med 8 352ra108 (2016)
PMID: 27535619

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171593 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase 3 36kDa subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 35.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13188 (Residues: 15-313; Coverage: 61%)
Gene names: KRS1, MST2, STK3
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand 5BS 2 x 5BS
Ligand CL 1 x CL
Ligand SO4 1 x SO4
1 modified residue:
Ligand TPO 4 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: C2221
Unit cell:
a: 89.871Å b: 98.462Å c: 166.771Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.211 0.247
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

38 review citations

Hippo-YAP/TAZ signalling in organ regeneration and regenerative medicine.
Moya et al. (2019)
37 more

2 mentions without citation

Dimerization and autophosphorylation of the MST family of kinases are controlled by the same set of residues.
Weingartner et al. (2023)
1 more