› 5dk2

X-ray diffraction
2.6Å resolution

Fc Heterodimer E356K/D399K + K392D/K409D

Released:
DOI: 10.2210/pdb5dk2/pdb
PDB entry 5dk2 coloured by chain, front view.
PDB entry 5dk2 coloured by chain, side view.
PDB entry 5dk2 coloured by chain, top view.
Source organisms:
Primary publication:
Computationally Designed Bispecific Antibodies using Negative State Repertoires.
Leaver-Fay A, Froning KJ, Atwell S, Aldaz H, Pustilnik A, Lu F, Huang F, Yuan R, Hassanali S, Chamberlain AK, Fitchett JR, Demarest SJ, Kuhlman B
Structure 24 641-651 (2016)
PMID: 26996964

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-134828 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Immunoglobulin heavy constant gamma 1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 227 amino acids
Theoretical weight: 25.63 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01857 (Residues: 104-328; Coverage: 56%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Immunoglobulin heavy constant gamma 1 Chains: B, E
Molecule details ›
Chains: B, E
Length: 240 amino acids
Theoretical weight: 26.95 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P01857 (Residues: 104-328; Coverage: 56%)
Gene name: IGHG1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Fc-III peptide Chain: C
Molecule details ›
Chain: C
Length: 13 amino acids
Theoretical weight: 1.53 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN, GAL
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 64.857Å b: 60.565Å c: 159.448Å
α: 90° β: 97.81° γ: 90°
R-values:
R R work R free
0.228 0.225 0.289
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

10 review citations

The making of bispecific antibodies.
Brinkmann et al. (2017)
9 more

4 mentions without citation

Immunoglobulin Fc Heterodimer Platform Technology: From Design to Applications in Therapeutic Antibodies and Proteins.
Ha et al. (2016)
3 more