› 5dmz

X-ray diffraction
2.4Å resolution

Structure of human Bub1 kinase domain phosphorylated at Ser969

Released:
DOI: 10.2210/pdb5dmz/pdb
PDB entry 5dmz coloured by chain, front view.
PDB entry 5dmz coloured by chain, side view.
PDB entry 5dmz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Role of Intrinsic and Extrinsic Factors in the Regulation of the Mitotic Checkpoint Kinase Bub1.
Breit C, Bange T, Petrovic A, Weir JR, Müller F, Vogt D, Musacchio A
PLoS One 10 e0144673 (2015)
PMID: 26658523

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129061 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitotic checkpoint serine/threonine-protein kinase BUB1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 365 amino acids
Theoretical weight: 41.92 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: O43683 (Residues: 726-1085; Coverage: 33%)
Gene names: BUB1, BUB1L
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 2 x ADP
Ligand MG 2 x MG
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 62.38Å b: 66.34Å c: 106.54Å
α: 90° β: 98.34° γ: 90°
R-values:
R R work R free
0.212 0.21 0.245
Expression system: Trichoplusia ni

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Citations

2 review citations

Mechanisms of Mitotic Kinase Regulation: A Structural Perspective.
Welburn et al. (2018)
1 more

1 mention without citation

Role of Intrinsic and Extrinsic Factors in the Regulation of the Mitotic Checkpoint Kinase Bub1.
Breit et al. (2015)