› 5do7

X-ray diffraction
3.93Å resolution

Crystal Structure of the Human Sterol Transporter ABCG5/ABCG8

Released:
DOI: 10.2210/pdb5do7/pdb
PDB entry 5do7 coloured by chain, front view.
PDB entry 5do7 coloured by chain, side view.
PDB entry 5do7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the human sterol transporter ABCG5/ABCG8.
Lee JY, Kinch LN, Borek DM, Wang J, Wang J, Urbatsch IL, Xie XS, Grishin NV, Cohen JC, Otwinowski Z, Hobbs HH, Rosenbaum DM
Nature 533 561-4 (2016)
PMID: 27144356

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190524 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ATP-binding cassette sub-family G member 5 Chains: A, C
Molecule details ›
Chains: A, C
Length: 666 amino acids
Theoretical weight: 74.51 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9H222 (Residues: 1-651; Coverage: 100%)
Gene name: ABCG5
Sequence domains:
ATP-binding cassette sub-family G member 8 Chains: B, D
Molecule details ›
Chains: B, D
Length: 685 amino acids
Theoretical weight: 76.99 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9H221 (Residues: 2-673; Coverage: 100%)
Gene name: ABCG8
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID, APS BEAMLINE 23-ID-D
Spacegroup: I222
Unit cell:
a: 173.552Å b: 224.804Å c: 253.304Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.245 0.329
Expression system: Komagataella pastoris

Quick links

Citations

30 review citations

Mechanisms and regulation of cholesterol homeostasis.
Luo et al. (2020)
29 more

34 mentions without citation

Structural and functional diversity calls for a new classification of ABC transporters.
Thomas et al. (2020)
33 more