› 5dsv

X-ray diffraction
3.75Å resolution

Crystal structure of human proteasome alpha7 tetradecamer

Released:
DOI: 10.2210/pdb5dsv/pdb
PDB entry 5dsv coloured by chain, front view.
PDB entry 5dsv coloured by chain, side view.
PDB entry 5dsv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Disassembly of the self-assembled, double-ring structure of proteasome α7 homo-tetradecamer by α6.
Ishii K, Noda M, Yagi H, Thammaporn R, Seetaha S, Satoh T, Kato K, Uchiyama S
Sci Rep 5 18167 (2015)
PMID: 26657688

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetradecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150582 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proteasome subunit alpha type-3 Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 255 amino acids
Theoretical weight: 28.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25788 (Residues: 1-255; Coverage: 100%)
Gene names: HC8, PSC8, PSMA3
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P43212
Unit cell:
a: 132.481Å b: 132.481Å c: 444.563Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.209 0.291
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Proteasome Structure and Assembly.
Budenholzer et al. (2017)
1 more

6 mentions without citation

Stereochemistry and Validation of Macromolecular Structures.
Wlodawer A. (2017)
5 more