› 5dwp

X-ray diffraction
1.2Å resolution

Crystal Structure of human transthyretin (TTR) processed with the CrystalDirect automated mounting and cryo-cooling technology

Released:
DOI: 10.2210/pdb5dwp/pdb
PDB entry 5dwp coloured by chain, front view.
PDB entry 5dwp coloured by chain, side view.
PDB entry 5dwp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Automated harvesting and processing of protein crystals through laser photoablation.
Zander U, Hoffmann G, Cornaciu I, Marquette JP, Papp G, Landret C, Seroul G, Sinoir J, Röwer M, Felisaz F, Rodriguez-Puente S, Mariaule V, Murphy P, Mathieu M, Cipriani F, Márquez JA
Acta Crystallogr D Struct Biol 72 454-66 (2016)
PMID: 27050125

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136168 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transthyretin Chains: A, B
Molecule details ›
Chains: A, B
Length: 116 amino acids
Theoretical weight: 12.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P02766 (Residues: 30-145; Coverage: 91%)
Gene names: PALB, TTR
Sequence domains: HIUase/Transthyretin family
Structure domains: Transthyretin/hydroxyisourate hydrolase domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Unit cell:
a: 41.346Å b: 62.521Å c: 85.427Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.163 0.196
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

2 review citations

Protein X-ray Crystallography and Drug Discovery.
Maveyraud et al. (2020)
1 more

1 mention without citation

Automated harvesting and processing of protein crystals through laser photoablation.
Zander et al. (2016)