› 5dzd

X-ray diffraction
1.57Å resolution

Crystal Structure of WW4 domain of ITCH in complex with TXNIP peptide

Released:
DOI: 10.2210/pdb5dzd/pdb
PDB entry 5dzd coloured by chain, front view.
PDB entry 5dzd coloured by chain, side view.
PDB entry 5dzd coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Liu Y, Tempel W, Dong A, Bountra C, Arrowsmith CH, Edwards AM, Min J, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188557 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase Itchy homolog Chains: A, B
Molecule details ›
Chains: A, B
Length: 47 amino acids
Theoretical weight: 5.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96J02 (Residues: 475-514; Coverage: 4%)
Gene name: ITCH
Sequence domains: WW domain
Thioredoxin-interacting protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 14 amino acids
Theoretical weight: 1.36 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9H3M7 (Residues: 327-338; Coverage: 3%)
Gene names: TXNIP, VDUP1

Ligands and Environments

1 bound ligand:
Ligand UNX 17 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P43212
Unit cell:
a: 52.668Å b: 52.668Å c: 82.476Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.175 0.206
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links