› 5e0t

X-ray diffraction
2.67Å resolution

Human PCNA mutant - S228I

Released:
DOI: 10.2210/pdb5e0t/pdb
PDB entry 5e0t coloured by chain, front view.
PDB entry 5e0t coloured by chain, side view.
PDB entry 5e0t coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A Disease-Causing Variant in PCNA Disrupts a Promiscuous Protein Binding Site.
Duffy CM, Hilbert BJ, Kelch BA
J Mol Biol 428 1023-1040 (2016)
PMID: 26688547

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-146041 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proliferating cell nuclear antigen Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 261 amino acids
Theoretical weight: 28.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P12004 (Residues: 1-261; Coverage: 100%)
Gene name: PCNA
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P43212
Unit cell:
a: 162.324Å b: 162.324Å c: 139.837Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.189 0.226
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Forging Ahead through Darkness: PCNA, Still the Principal Conductor at the Replication Fork.
Choe et al. (2017)
2 more

1 mention without citation

A thermosensitive PCNA allele underlies an ataxia-telangiectasia-like disorder.
Magrino et al. (2023)