› 5e0u

X-ray diffraction
1.93Å resolution

Human PCNA variant (S228I) complexed with p21 at 1.9 Angstroms

Released:
DOI: 10.2210/pdb5e0u/pdb
PDB entry 5e0u coloured by chain, front view.
PDB entry 5e0u coloured by chain, side view.
PDB entry 5e0u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A Disease-Causing Variant in PCNA Disrupts a Promiscuous Protein Binding Site.
Duffy CM, Hilbert BJ, Kelch BA
J Mol Biol 428 1023-1040 (2016)
PMID: 26688547

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146037 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proliferating cell nuclear antigen Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 261 amino acids
Theoretical weight: 28.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P12004 (Residues: 1-261; Coverage: 100%)
Gene name: PCNA
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen
Cyclin-dependent kinase inhibitor 1 Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 23 amino acids
Theoretical weight: 2.88 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P38936 (Residues: 139-160; Coverage: 13%)
Gene names: CAP20, CDKN1, CDKN1A, CIP1, MDA6, PIC1, SDI1, WAF1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P3
Unit cell:
a: 143.295Å b: 143.295Å c: 41.402Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.162 0.221
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Forging Ahead through Darkness: PCNA, Still the Principal Conductor at the Replication Fork.
Choe et al. (2017)
2 more

7 mentions without citation

Cloning, recombinant production and crystallographic structure of Proliferating Cell Nuclear Antigen from radioresistant archaeon Thermococcus gammatolerans.
Venancio-Landeros et al. (2016)
6 more