› 5e0v

X-ray diffraction
2.07Å resolution

Human PCNA variant (S228I) complexed with FEN1 at 2.1 Angstroms

Released:
DOI: 10.2210/pdb5e0v/pdb
PDB entry 5e0v coloured by chain, front view.
PDB entry 5e0v coloured by chain, side view.
PDB entry 5e0v coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A Disease-Causing Variant in PCNA Disrupts a Promiscuous Protein Binding Site.
Duffy CM, Hilbert BJ, Kelch BA
J Mol Biol 428 1023-1040 (2016)
PMID: 26688547

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146038 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proliferating cell nuclear antigen Chains: A, B
Molecule details ›
Chains: A, B
Length: 261 amino acids
Theoretical weight: 28.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P12004 (Residues: 1-261; Coverage: 100%)
Gene name: PCNA
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen
Flap endonuclease 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 16 amino acids
Theoretical weight: 1.77 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P39748 (Residues: 335-350; Coverage: 4%)
Gene names: FEN1, RAD2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P3
Unit cell:
a: 80.721Å b: 80.721Å c: 66.556Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.216 0.202 0.229
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Forging Ahead through Darkness: PCNA, Still the Principal Conductor at the Replication Fork.
Choe et al. (2017)
2 more