› 5e3h

X-ray diffraction
2.7Å resolution

Structural Basis for RNA Recognition and Activation of RIG-I

Released:
DOI: 10.2210/pdb5e3h/pdb
PDB entry 5e3h coloured by chain, front view.
PDB entry 5e3h coloured by chain, side view.
PDB entry 5e3h coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of RNA recognition and activation by innate immune receptor RIG-I.
Jiang F, Ramanathan A, Miller MT, Tang GQ, Gale M, Patel SS, Marcotrigiano J
Nature 479 423-7 (2011)
PMID: 21947008

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132025 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Antiviral innate immune response receptor RIG-I Chain: A
Molecule details ›
Chain: A
Length: 695 amino acids
Theoretical weight: 79.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95786 (Residues: 232-925; Coverage: 75%)
Gene names: DDX58, RIGI
Sequence domains:
Structure domains:
RNA (5'-R(*CP*GP*AP*CP*GP*CP*UP*AP*GP*CP*GP*U)-3') Chains: B, C
Molecule details ›
Chains: B, C
Length: 14 nucleotides
Theoretical weight: 4.48 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

5 bound ligands:
Ligand ZN 1 x ZN
Ligand MG 3 x MG
Ligand GOL 1 x GOL
Ligand ADP 1 x ADP
Ligand BEF 1 x BEF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P6522
Unit cell:
a: 176.03Å b: 176.03Å c: 108.071Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.236 0.234 0.268
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

103 review citations

Signaling in innate immunity and inflammation.
Newton et al. (2012)
102 more

14 mentions without citation

Discrimination of cytosolic self and non-self RNA by RIG-I-like receptors.
Lässig et al. (2017)
13 more