› 5e5b

X-ray diffraction
1.84Å resolution

Crystal structure of Human Spt16 N-terminal domain

Released:
DOI: 10.2210/pdb5e5b/pdb
PDB entry 5e5b coloured by chain, front view.
PDB entry 5e5b coloured by chain, side view.
PDB entry 5e5b coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the human histone chaperone FACT Spt16 N-terminal domain.
Marcianò G, Huang DT
Acta Crystallogr F Struct Biol Commun 72 121-8 (2016)
PMID: 26841762

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195268 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FACT complex subunit SPT16 Chain: A
Molecule details ›
Chain: A
Length: 431 amino acids
Theoretical weight: 48.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y5B9 (Residues: 2-432; Coverage: 41%)
Gene names: FACT140, FACTP140, SUPT16H
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: F432
Unit cell:
a: 246.57Å b: 246.57Å c: 246.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.156 0.176
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Fly Fishing for Histones: Catch and Release by Histone Chaperone Intrinsically Disordered Regions and Acidic Stretches.
Warren et al. (2017)
1 more

6 mentions without citation

Structure-function relationship of H2A-H2B specific plant histone chaperones.
Kumar et al. (2020)
5 more