› 5e8d

X-ray diffraction
2.5Å resolution

Crystal structure of human epiregulin in complex with the Fab fragment of murine monoclonal antibody 9E5

Released:
DOI: 10.2210/pdb5e8d/pdb
PDB entry 5e8d coloured by chain, front view.
PDB entry 5e8d coloured by chain, side view.
PDB entry 5e8d coloured by chain, top view.
Source organisms:
Primary publication:
Epiregulin Recognition Mechanisms by Anti-Epiregulin Antibody 9E5: Structural, Functional and Molecular Dynamics Simulation Analyses.
Kado Y, Mizohata E, Nagatoishi S, Iijima M, Shinoda K, Miyafusa T, Nakayama T, Yoshizumi T, Sugiyama A, Kawamura T, Lee YH, Matsumura H, Doi H, Fujitani H, Kodama T, Shibasaki Y, Tsumoto K, Inoue T
J. Biol. Chem. (2015)
PMID: 26627827

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208043 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Proepiregulin Chain: A
Molecule details ›
Chain: A
Length: 75 amino acids
Theoretical weight: 8.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14944 (Residues: 38-108; Coverage: 51%)
Gene name: EREG
Structure domains: Laminin
anti-human epiregulin antibody 9E5 Fab heavy chain Chain: H
Molecule details ›
Chain: H
Length: 220 amino acids
Theoretical weight: 23.53 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
anti-human epiregulin antibody 9E5 Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 23.61 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: I222
Unit cell:
a: 68.602Å b: 100.291Å c: 187.369Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.19 0.263
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Heavy Atom Detergent/Lipid Combined X-ray Crystallography for Elucidating the Structure-Function Relationships of Membrane Proteins.
Hanashima et al. (2021)
5 other articles

3 mentions without citation

EGFR Ligands Differentially Stabilize Receptor Dimers to Specify Signaling Kinetics.
Freed et al. (2017)
2 more