› 5e94

X-ray diffraction
2Å resolution

Antibody-bound Glucagon-like Peptide-1 receptor extracellular domain

Released:
DOI: 10.2210/pdb5e94/pdb
PDB entry 5e94 coloured by chain, front view.
PDB entry 5e94 coloured by chain, side view.
PDB entry 5e94 coloured by chain, top view.
Source organisms:
Primary publication:
Structural insight into antibody-mediated antagonism of the Glucagon-like peptide-1 Receptor.
Hennen S, Kodra JT, Soroka V, Krogh BO, Wu X, Kaastrup P, Ørskov C, Rønn SG, Schluckebier G, Barbateskovic S, Gandhi PS, Reedtz-Runge S
Sci Rep 6 26236 (2016)
PMID: 27196125

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211238 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Antibody Fab fragment light chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 214 amino acids
Theoretical weight: 23.52 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Antibody Fab fragment heavy chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 224 amino acids
Theoretical weight: 24.43 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Glucagon-like peptide 1 receptor Chains: G, H
Molecule details ›
Chains: G, H
Length: 126 amino acids
Theoretical weight: 14.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P43220 (Residues: 24-145; Coverage: 28%)
Gene name: GLP1R
Sequence domains: Hormone receptor domain
Structure domains: GPCR, family 2, extracellular hormone receptor domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P1
Unit cell:
a: 57.201Å b: 65.692Å c: 88.351Å
α: 111.61° β: 97.47° γ: 91.28°
R-values:
R R work R free
0.19 0.188 0.232
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

5 review citations

Opportunities for therapeutic antibodies directed at G-protein-coupled receptors.
Hutchings et al. (2017)
4 more

6 mentions without citation

Structural insight into antibody-mediated antagonism of the Glucagon-like peptide-1 Receptor.
Hennen et al. (2016)
5 more