› 5eaw

X-ray diffraction
3Å resolution

Crystal structure of Dna2 nuclease-helicase

Released:
DOI: 10.2210/pdb5eaw/pdb
PDB entry 5eaw coloured by chain, front view.
PDB entry 5eaw coloured by chain, side view.
PDB entry 5eaw coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Dna2 nuclease-helicase structure, mechanism and regulation by Rpa.
Zhou C, Pourmal S, Pavletich NP
Elife 4 (2015)
PMID: 26491943

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180597 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA replication ATP-dependent helicase/nuclease DNA2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 1056 amino acids
Theoretical weight: 118.93 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q6ZQJ5 (Residues: 1-1056; Coverage: 99%)
Gene names: Dna2, Dna2l, Kiaa0083
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand SF4 2 x SF4
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 120.9Å b: 148.6Å c: 170.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.209 0.247
Expression system: Spodoptera frugiperda

Quick links

Citations

20 review citations

DNA Repair Pathway Choices in CRISPR-Cas9-Mediated Genome Editing.
Xue et al. (2021)
19 more

4 mentions without citation

TWINKLE and Other Human Mitochondrial DNA Helicases: Structure, Function and Disease.
Peter et al. (2020)
3 more