› 5ecj

X-ray diffraction
3.05Å resolution

Crystal structure of monobody Mb(S4) bound to Prdm14 in complex with Mtgr1

Released:
DOI: 10.2210/pdb5ecj/pdb
PDB entry 5ecj coloured by chain, front view.
PDB entry 5ecj coloured by chain, side view.
PDB entry 5ecj coloured by chain, top view.
Source organisms:
Primary publication:
ETO family protein Mtgr1 mediates Prdm14 functions in stem cell maintenance and primordial germ cell formation.
Nady N, Gupta A, Ma Z, Swigut T, Koide A, Koide S, Wysocka J
Elife 4 e10150 (2015)
PMID: 26523391

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123213 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PR domain zinc finger protein 14; Protein CBFA2T2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 309 amino acids
Theoretical weight: 34.52 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: E9Q3T6 (Residues: 184-373; Coverage: 34%)
  • Canonical: O70374 (Residues: 98-206; Coverage: 18%)
Gene names: Cbfa2t2, Cbfa2t2h, Mtgr1, Prdm14
Sequence domains:
Monobody Mb(S4) Chains: E, F
Molecule details ›
Chains: E, F
Length: 95 amino acids
Theoretical weight: 10.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43212
Unit cell:
a: 106.812Å b: 106.812Å c: 180.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.189 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Specification and epigenetic programming of the human germ line.
Tang et al. (2016)
10 more

3 mentions without citation

The duality of PRDM proteins: epigenetic and structural perspectives.
Di Tullio et al. (2022)
2 more