› 5eib

X-ray diffraction
2.1Å resolution

Crystal structure of CPAP PN2-3 C-terminal loop-helix in complex with DARPin-tubulin

Released:
DOI: 10.2210/pdb5eib/pdb
PDB entry 5eib coloured by chain, front view.
PDB entry 5eib coloured by chain, side view.
PDB entry 5eib coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis for CPAP-tubulin interaction in controlling centriolar and ciliary length.
Zheng X, Ramani A, Soni K, Gottardo M, Zheng S, Ming Gooi L, Li W, Feng S, Mariappan A, Wason A, Widlund P, Pozniakovsky A, Poser I, Deng H, Ou G, Riparbelli M, Giuliano C, Hyman AA, Sattler M, Gopalakrishnan J, Li H
Nat Commun 7 11874 (2016)
PMID: 27306797

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-160563 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha-1B chain Chain: C
Molecule details ›
Chain: C
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P81947 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Structure domains:
Tubulin beta-2B chain Chain: D
Molecule details ›
Chain: D
Length: 445 amino acids
Theoretical weight: 49.98 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:
Structure domains:
Designed ankyrin repeat protein Chain: E
Molecule details ›
Chain: E
Length: 169 amino acids
Theoretical weight: 18 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain
Centromere protein J Chain: F
Molecule details ›
Chain: F
Length: 23 amino acids
Theoretical weight: 2.69 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9HC77 (Residues: 372-394; Coverage: 2%)
Gene names: CENPJ, CPAP, LAP, LIP1

Ligands and Environments

2 bound ligands:
Ligand GTP 2 x GTP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 73.856Å b: 91.116Å c: 83.31Å
α: 90° β: 96.97° γ: 90°
R-values:
R R work R free
0.18 0.178 0.222
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

11 review citations

Once and only once: mechanisms of centriole duplication and their deregulation in disease.
Nigg et al. (2018)
10 more

3 mentions without citation

Molecular basis for CPAP-tubulin interaction in controlling centriolar and ciliary length.
Zheng et al. (2016)
2 more