› 5eii

X-ray diffraction
2.44Å resolution

Structural determination of an protein complex of a Fab with increased solubility

Released:
DOI: 10.2210/pdb5eii/pdb
PDB entry 5eii coloured by chain, front view.
PDB entry 5eii coloured by chain, side view.
PDB entry 5eii coloured by chain, top view.
Source organisms:
Primary publication:
Structural determination of an protein complex of a Fab with increased solubility
Bailey LJ, Kossiakoff AA, Schaefer ZP
To be published

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211067 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab Heavy Chain Chains: A, H
Molecule details ›
Chains: A, H
Length: 235 amino acids
Theoretical weight: 25.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab Light Chain Chains: B, L
Molecule details ›
Chains: B, L
Length: 215 amino acids
Theoretical weight: 23.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Histone chaperone ASF1 Chains: G, I
Molecule details ›
Chains: G, I
Length: 156 amino acids
Theoretical weight: 17.75 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P32447 (Residues: 1-156; Coverage: 56%)
Gene names: ASF1, CIA1, J0755, YJL115W
Sequence domains: ASF1 like histone chaperone
Structure domains: Histone chaperone ASF1-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 81.486Å b: 62.945Å c: 161.828Å
α: 90° β: 94° γ: 90°
R-values:
R R work R free
0.188 0.185 0.233
Expression system: Escherichia coli

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