› 5eir

X-ray diffraction
2.69Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139175 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Eukaryotic translation initiation factor 4E Chain: A
Molecule details ›
Chain: A
Length: 217 amino acids
Theoretical weight: 25.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06730 (Residues: 1-217; Coverage: 100%)
Gene names: EIF4E, EIF4EL1, EIF4F
Sequence domains: Eukaryotic initiation factor 4E
Structure domains: RNA Cap, Translation Initiation Factor Eif4e
Eukaryotic translation initiation factor 4 gamma 1 Chain: B
Molecule details ›
Chain: B
Length: 14 amino acids
Theoretical weight: 1.85 KDa
Source organism: synthetic construct
Expression system: Not provided
UniProt:
  • Canonical: Q04637 (Residues: 609-622; Coverage: 1%)
Gene names: EIF4F, EIF4G, EIF4G1, EIF4GI

Ligands and Environments

2 bound ligands:
Ligand 5O8 1 x 5O8
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Unit cell:
a: 38.25Å b: 52.12Å c: 123.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.249 0.248 0.272
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

RNA-modifying proteins as anticancer drug targets.
Boriack-Sjodin et al. (2018)
12 other articles

1 mention without citation

Design of nucleotide-mimetic and non-nucleotide inhibitors of the translation initiation factor eIF4E: Synthesis, structural and functional characterisation.
Soukarieh et al. (2016)