› 5eiz

X-ray diffraction
1.96Å resolution

Crystal structure of Y99A mutant of human macrophage migration inhibitory factor

Released:
DOI: 10.2210/pdb5eiz/pdb
PDB entry 5eiz coloured by chain, front view.
PDB entry 5eiz coloured by chain, side view.
PDB entry 5eiz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Nanosecond Dynamics Regulate the MIF-Induced Activity of CD74.
Pantouris G, Ho J, Shah D, Syed MA, Leng L, Bhandari V, Bucala R, Batista VS, Loria JP, Lolis EJ
Angew Chem Int Ed Engl 57 7116-7119 (2018)
PMID: 29669180

Function and Biology Details

Reactions catalysed: 
Keto-phenylpyruvate = enol-phenylpyruvate
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146863 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage migration inhibitory factor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 114 amino acids
Theoretical weight: 12.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14174 (Residues: 2-115; Coverage: 99%)
Gene names: GLIF, MIF, MMIF
Sequence domains: Macrophage migration inhibitory factor (MIF)
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

3 bound ligands:
Ligand GOL 3 x GOL
Ligand SO4 3 x SO4
Ligand IPA 2 x IPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 67.696Å b: 67.958Å c: 86.519Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.157 0.181
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Molecular Level Insights Into the Structural and Dynamic Factors Driving Cytokine Function.
Cui et al. (2021)
13 other articles

1 mention without citation

Regulation of MIF Enzymatic Activity by an Allosteric Site at the Central Solvent Channel.
Pantouris et al. (2020)