› 5ekv

X-ray diffraction
3.61Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139176 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Eukaryotic translation initiation factor 4E Chains: A, C
Molecule details ›
Chains: A, C
Length: 217 amino acids
Theoretical weight: 25.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06730 (Residues: 1-217; Coverage: 100%)
Gene names: EIF4E, EIF4EL1, EIF4F
Sequence domains: Eukaryotic initiation factor 4E
Structure domains: RNA Cap, Translation Initiation Factor Eif4e
Eukaryotic translation initiation factor 4E-binding protein 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 14 amino acids
Theoretical weight: 1.86 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q13541 (Residues: 51-64; Coverage: 12%)
Gene name: EIF4EBP1

Ligands and Environments

1 bound ligand:
Ligand 5PQ 2 x 5PQ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 38.48Å b: 100.78Å c: 136.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.27 0.266 0.342
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

RNA-modifying proteins as anticancer drug targets.
Boriack-Sjodin et al. (2018)
12 other articles

2 mentions without citation

Design of nucleotide-mimetic and non-nucleotide inhibitors of the translation initiation factor eIF4E: Synthesis, structural and functional characterisation.
Soukarieh et al. (2016)
1 more