› 5eof

X-ray diffraction
2.05Å resolution

Crystal structure of OPTN NTD and TBK1 CTD complex

Released:
DOI: 10.2210/pdb5eof/pdb
PDB entry 5eof coloured by chain, front view.
PDB entry 5eof coloured by chain, side view.
PDB entry 5eof coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into the interaction and disease mechanism of neurodegenerative disease-associated optineurin and TBK1 proteins.
Li F, Xie X, Wang Y, Liu J, Cheng X, Guo Y, Gong Y, Hu S, Pan L
Nat Commun 7 12708 (2016)
PMID: 27620379

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188422 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Optineurin Chains: A, B
Molecule details ›
Chains: A, B
Length: 82 amino acids
Theoretical weight: 9.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96CV9 (Residues: 26-103; Coverage: 14%)
Gene names: FIP2, GLC1E, HIP7, HYPL, NRP, OPTN
Sequence domains: NF-kappa-B essential modulator NEMO
Serine/threonine-protein kinase TBK1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 57 amino acids
Theoretical weight: 6.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UHD2 (Residues: 677-729; Coverage: 7%)
Gene names: NAK, TBK1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 36.321Å b: 54.775Å c: 153.141Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.208 0.245
Expression system: Escherichia coli

Quick links

Citations

29 review citations

Biological Functions of Autophagy Genes: A Disease Perspective.
Levine et al. (2019)
28 more

5 mentions without citation

The role of protein complexes in human genetic disease.
Bergendahl et al. (2019)
4 more