› 5epg

X-ray diffraction
3.39Å resolution

Human aldehyde oxidase SNP S1271L

Released:
DOI: 10.2210/pdb5epg/pdb
PDB entry 5epg coloured by chain, front view.
PDB entry 5epg coloured by chain, side view.
PDB entry 5epg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Optimization of the Expression of Human Aldehyde Oxidase for Investigations of Single-Nucleotide Polymorphisms.
Foti A, Hartmann T, Coelho C, Santos-Silva T, Romão MJ, Leimkühler S
Drug Metab Dispos 44 1277-85 (2016)
PMID: 26842593

Function and Biology Details

Reaction catalysed: 
An aldehyde + H(2)O + O(2) = a carboxylate + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170321 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldehyde oxidase Chain: A
Molecule details ›
Chain: A
Length: 1338 amino acids
Theoretical weight: 148.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06278 (Residues: 1-1338; Coverage: 100%)
Gene names: AO, AOX1
Sequence domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD

Cofactor: Ligand MTE 1 x MTE
2 bound ligands:
Ligand FES 2 x FES
Ligand MOS 1 x MOS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P42212
Unit cell:
a: 147.439Å b: 147.439Å c: 131.713Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.162 0.253
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Evolution, expression, and substrate specificities of aldehyde oxidase enzymes in eukaryotes.
Terao et al. (2020)
3 more

3 mentions without citation

Human aldehyde oxidase (hAOX1): structure determination of the Moco-free form of the natural variant G1269R and biophysical studies of single nucleotide polymorphisms.
Mota et al. (2019)
2 more