› 5es3

X-ray diffraction
2.29Å resolution

Co-crystal structure of LDH liganded with oxamate

Released:
DOI: 10.2210/pdb5es3/pdb
PDB entry 5es3 coloured by chain, front view.
PDB entry 5es3 coloured by chain, side view.
PDB entry 5es3 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
A Streamlined, Automated Protocol for the Production of Milligram Quantities of Untagged Recombinant Rat Lactate Dehydrogenase A Using ÄKTAxpressTM.
Nowicki MW, Blackburn EA, McNae IW, Wear MA
PLoS One 10 e0146164 (2015)
PMID: 26717415

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138140 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase A chain Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 331 amino acids
Theoretical weight: 36.36 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04642 (Residues: 2-332; Coverage: 100%)
Gene names: Ldh-1, Ldh1, Ldha
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand OXM 8 x OXM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 84.08Å b: 146.63Å c: 284.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.17 0.203
Expression system: Escherichia coli

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Citations

2 review citations

The fed-batch principle for the molecular biology lab: controlled nutrient diets in ready-made media improve production of recombinant proteins in Escherichia coli.
Krause et al. (2016)
1 more

1 mention without citation

A Streamlined, Automated Protocol for the Production of Milligram Quantities of Untagged Recombinant Rat Lactate Dehydrogenase A Using ÄKTAxpressTM.
Nowicki et al. (2015)